Progress in high-throughput in vitro testing

In vitro assays and alternative approaches being developed for EPA’s Endocrine Disruptor Screening Program and within EU-ToxRisk are hitting ground

Alternative toxicity testing in the U.S.

U.S. EPA publishes draft strategy for use of alternative toxicity tests in evaluation of chemicals under updated chemicals regulation

Alternative plasticizers adipogenic in vitro

Several alternative plasticizers used in FCMs shown to promote lipid accumulation in vitro; direct activation of PPARγ suggested as possible mechanism

In vitro tests for toxicological profiling of FCMs

In vitro assays demonstrate significant biological activity in extracts from diverse food contact articles made of paper and board; bioassays expected to become accepted tool for assessment of FCM safety

Microbiome and chemical risk assessment

U.S. National Academy of Sciences’ report outlines research strategy to assess the need for chemical risk assessments to consider interactions between chemicals and microbiota when estimating human health effects

Regulatory status of non-animal approaches

U.S. NAS workshop suggests comparing non-animal tests to human, not rodent, data, predicts alternatives to soon replace whole-animal tests; ECHA sees “long-way” until regulatory acceptance of alternatives for complex endpoints

Utility of bioassays in safety assessment

Scientists demonstrate utility of bioassays in FCM safety assessment using 4-nonylphenol (4NP) as model substance; 4NP levels detected by bioassays considered relevant for safety assessment

In vitro testing of paper and board FCMs

Scientists test paper and board FCMs in 8 in vitro bioassays; all 20 samples showed positive responses in at least one of the assays; chemicals in paper and board FCMs require further investigation to ensure safety